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Trehalose Review Article

Trehalose Review Articles

We use trehalose to stabilize some of our enzymes during a freeze-drying process. We used to purchase trehalose dihydrate from a well-known St. Louis company. However, quality was not consistent, forcing us to screen lots for the presence RNase which can compromise our products. Sometimes there was not lot available that would meet our criteria. Now, we process our own trehalose, both powder and sterile solution for internal use and for sale.

Stabilization, Freeze-Drying, etc.

Enzyme and antibody stabilization is our main interest. Other applications for cells and tissues are beyond our experience, but for those who are interested here is a short list of review articles.

  • Chen T et al.: (2000) Literature review: supplemented phase diagram of the trehalose-water binary mixture. Cryobiology v40 pp277-282.
  • Chen, Y and Z G Lu (2006) Research progress on trehalose used in lyophilization of blood cells – review. Zhongguo Shi Yan Xue Ye Xue Za Zhi v.14 pp 416-418.
  • Crowe, J H et al (2003) Stabilization of membranes in human platelets freeze-dried with trehalose., Chemistry and Physics of Lipids. V.122, no.1-2 pp 41-52.
  • Elbein, A D et al. (2003) Trehalose: a review of properties, history of use and human tolerance, and results of multiple safety studies. Glycobiology v. 13 no. 4 pp. 17R-27R
  • Elbein, A D et al. (2003) Review: New insights on trehalose: a multifunctional molecule. Glycobiology, v13 (4j) 17R-27R
  • 7. Franzetti , A et al. (2010) Production and Applications of Trehalose Lipid Biosurfactants. European Journal of Lipid Science and Technology. v.9999 no. 999A
  • Iturriaga, G et al. (2009) Trehalose Metabolism: From Osmoprotection to Signaling. International Journal of Molecular Sciences v.10 no.9, pp 3793-3810;
  • Jain N K and I Roy (2009) Effect of trehalose on protein structure. Protein Science v.18 pp 24-36
  • Richards, A B et al. (2002) Trehalose: a review of properties, history of use and human tolerance, and results of multiple safety studies. Food and Chemical Toxicology v.40 no.7 pp.871-98.
  • Ryll, R et al. (2001) Immunological properties of trehalose dimycolate (cord factor) and other mycolic acid-containing glycolipids–a review. Microbiology and Immunology v.45
  • Schwegman, J J et al. (2005) Practical Formulation and Process Development of Freeze-Dried Products. Pharmaceutical Development and Technology v10 (2)151 – 173 A1
  • Teramoto , N et al. (2008) Trehalose and Trehalose-based Polymers for Environmentally Benign, Biocompatible and Bioactive Materials. Molecules v.13, no.8, 1773-1816
  • Ragoonanan, V and A Aksan (2007) Protein Stabilization. Transfusion Medicine and Hemotherapy v.34 pp 246–252
  • Molecular Biology Grade Trehalose

    Our trehalose dihydrate tests negative for RNase/DNase and endotoxin, plus its very low in other contaminants. Trehalose Solution is a 1M solution made from our Molecular Biology Grade and sterile filtered in a validated process. Full specifications and pricing may be found by following the links above.