Influenza A virus H5N1 RT-qPCR Kit
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Influenza A virus H5N1 RT-qPCR Kit
- Influenza A subtype H5N1 is a highly pathogenic avian influenza virus that primarily infects birds, particularly waterfowl, and has caused sporadic but severe outbreaks in poultry populations. This virus is of concern due to its potential to infect humans, causing severe respiratory illness, and has raised pandemic fears because of its high mortality rate when transmitted to people. Influenza A virus H5N1 RT-qPCR Kit is designed for the in vitro detection of Influenza A virus subtype H5N1 from clades 2.3.4.4b and 2.3.2.1a.
- The kit is built to have the broadest possible detection profile whilst remaining specific to Influenza A virus subtype H5N1. Thus, this kit has been designed for the specific (inclusivity) and exclusive (exclusivity) in vitro detection of this subtype. The primers and probe sequences have very high (>95%) homology with a broad range of Influenza A virus subtype H5N1 genomes based on a comprehensive bioinformatic analysis with all reference data within the NCBI and OpenFlu databases at the time of design. Due to the inherent instability of RNA viral genomes, it is not possible to guarantee the detection of all clinical isolates. This kit was meticulously designed and validated to meet the rigorous criteria of a quantitative assay. However, it is important to note that the provided Positive Control is not intended for quantification purposes.
- We recommend checking NZYtech website for the availability of a suitable Quantitative Standard for an accurate quantification. In alternative, commercially genomic RNA standards can also be used. If required, a complementary kit for the detection of an endogenous gene of the species from which samples are being extracted is available at NZYtech (see https://nzytech.com/en/molecular-diagnostics/). The complementary usage of an Endogenous Detection reaction provides a solid confirmation that nucleic acids were properly extracted from the selected biological matrix. If you require further information or have a specific question about the detection profile of this kit, please send an e-mail to info@nzytech.com and our scientific team will answer your question. This kit is designed to be used by trained users in a suitable molecular biology laboratory environment.
- Shipping: RT
- Storage: -85C to -15C
- Format: Lyophilized
- Product Category: Predesigned qPCR assays
- Compatibility: All standard Real-time thermal cycles
- Target Species: Influenza A virus H5N1
- Sample Material: RNA
- Protocol Time: Approx. 60 min. (from extracted NA to result)
- Number of Targets: Triplex
- Internal Control: No
- Detection Method: Probe
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